QE65000-FL Scientific-grade Spectrometer
for
Fluorescence Measurements
The QE65000-FL Scientific-grade Spectrometer is a
preconfigured combination
of detector, optical bench and electronics technologies that
provides users with a remarkably sensitive system for demanding
low-light level applications such as fluorescence. The QE65000-FL can achieve up to
90% quantum efficiency with high signal-to-noise and rapid signal
processing speed. The QE65000-FL preconfigured spectrometer system
is available for $11549.
New! Optical Bench
Enhancements
The great performance of our QE65000-FL Spectrometer has been enhanced to provide even more value: lower
stray light, improved efficiency in the UV and Shortwave NIR, and better
unit-to-unit reproducibility.
We are also offering a proprietary low
BRDF bench coating to improve stray light performance and provide better
reproducibility in unit-to-unit performance.
The QE65000 is the most sensitive
spectrometer we have ever developed. The
Hamamatsu FFT-CCD detector used in the QE65000-FL provides 90% quantum
efficiency (defined as how efficiently a photon is converted to a
photo-electron). Most of our other detectors are linear CCDs but with this
“2D” area detector, we can bin (or sum) a vertical row of pixels, which
offers significant improvement in the signal-to-noise ratio
(>1000:1) and signal
processing speed of the detector compared with a linear CCD, where signals
are digitally added by an external circuit.
In our spectrometers with linear CCDs, the
slit’s width, not its height, regulates the amount of light entering the
bench because linear CCDs cannot efficiently collect the light from the
entire height of the slit. But in the QE65000, the 2D area detector can
better take advantage of the height of the slit and the additional light,
greatly improving system sensitivity.
Because the detector in the QE65000-FL is
back-thinned (or back-illuminated), it has great native response in the UV
and does not require the additional coatings that we typically apply to
other detectors for UV applications.
The QE65000-FL Spectrometer is
specifically configured for low-light
level applications such as fluorescence.
The TE-cooled (down to -15 °C) detector features low noise and low dark
signal, which enables low-light-level
detection and long integration times (analogous to the shutter speed of a camera) from 8
milliseconds to 15 minutes, with virtually no spectral distortion.
The QE65000-FL also has an onboard
programmable micro-controller for controlling the spectrometer and
accessories. You have access to 10 user-programmable digital inputs/outputs and a pulse generator for triggering other devices.
You can use the I/Os
to flash a lamp, stop/start a process, and send a message/alarm during the
spectrometer’s integration period. The spectrometer’s operating parameters
can be controlled through software. In fact, wavelength calibration
coefficients unique to each spectrometer are programmed into a memory chip
right on the spectrometer.
The QE65000-FL interfaces to a computer via USB 2.0 or
RS-232 serial port. Data unique to each
spectrometer are programmed into a memory chip on the QE65000; our
SpectraSuite Spectroscopy
Operating Software reads these values for easy setup and hot swapping
among computers, whether they run on Linux, Mac or Windows operating
systems.
The QE65000-FL is preconfigured with the
following, along with SpectraSuite spectrometer operating software and
our Annual Service Package (ASP) to extend the warranty from 1 year to 2
years:
|
Equipment |
Details |
|
Spectrometer |
QE65000 |
|
Grating |
HC1 |
|
Slit |
SLIT-200 |
|
Item |
Description |
Price |
|
QE65000-FL |
QE65000-FL preconfigured
spectrometer system. Includes SpectraSuite and an ASP. |
$12,835 |
|
Physical
|
|
Dimensions: |
182 mm x 110 mm x 47 mm |
|
Weight: |
1.18 kg (without power supply) |
|
Detector |
|
Detector:
|
Hamamatsu S7031-1006 |
|
Detector range: |
200-1100 nm |
|
Pixels:
|
1024 x 58 (1044 x 64 total pixels) |
|
Pixel size:
|
24.576 μm2 |
|
Pixel well depth:
|
1000 Ke- |
|
Sensitivity: |
~0.065 counts / e- |
|
Quantum efficiency: |
90% peak; 65% at 250 nm |
|
Optical Bench |
|
Design: |
f/4, Symmetrical crossed Czerny-Turner |
|
Focal length: |
101.6 mm input and output |
|
Entrance aperture: |
200 µm wide slit |
|
HC1-QE grating: |
provides 250-950 nm range |
|
OFLV filter: |
OFLV-QE-250
(250-1000 nm) |
|
Collimating and focusing mirrors: |
Standard only |
|
UV enhanced window: |
No |
|
Fiber optic connector: |
SMA 905 to 0.22 numerical aperture single-strand optical fiber |
|
Spectroscopic |
|
Wavelength range: |
250-950 (grating dependent) |
|
Optical resolution: |
~0.14-7.7 nm FWHM |
|
Signal-to-noise ratio: |
1000:1 (at full signal) |
|
A/D resolution: |
16 bit |
|
Dark noise: |
3 RMS counts |
|
Dynamic range: |
7.5 x 109
(system), 25000:1 for a single acquisition |
|
Integration time: |
8 ms to 15 minutes |
|
Stray light: |
<0.08% at 600 nm; 0.4% at 435 nm |
|
Corrected linearity: |
>99.8% |
|
Electronics |
|
Power consumption: |
500 mA @ 5 VDC (no TE cooling); 3.5 A @ 5 VDC (with
TE cooling) |
|
Data transfer speed: |
Full scans to memory every 7 ms with USB 2.0 port,
18 ms with USB1.1 port, 300 ms with serial port |
|
Inputs/Outputs: |
10 onboard digital user-programmable GPIOs (general purpose
inputs/outputs) |
|
Analog channels: |
No |
|
Auto nulling: |
Yes |
|
Breakout box compatibility: |
Yes,
HR4-BREAKOUT |
|
Trigger modes: |
4 modes |
|
Strobe functions: |
No |
|
Gated delay feature: |
Yes |
|
Connector: |
30-pin connector |
|
Power-up time: |
<5 seconds |
|
Dark current:
|
4000 e-/pixel/sec @ 25 ºC; 200
e-/pixel/sec @ 0 ºC |
|
Computer |
|
Operating systems: |
Windows 98/Me/2000/XP, Mac OS X and Linux with USB
port; Any 32-bit Windows OS with serial port |
|
Computer interfaces: |
USB 2.0 @ 480 Mbps; RS-232 (2-wire) @ 115.2 K baud |
|
Peripheral interfaces: |
SPI (3-wire); I2C inter-integrated circuit |
|
Temperature and Thermoelectric (TE) Cooling |
|
Temperature limits: |
0 ºC to 50.0 ºC; no condensation |
|
Set point: |
Software controlled; lowest set point is 40 ºC
below ambient |
|
Stability |
+/-0.1 ºC of set temperature in <2 minutes |
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