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| An example of a fluorescence setup with a QE65000 uses the PX-2 Pulsed Xenon Lamp as an excitation source and the CUV-ALL Cuvette Holder for samples. An optical fiber delivers excitation light to the sample holder and read light to the spectrometer. A filter, such as one of our Linear Variable Filters would block excitation light from entering the spectrometer |
The QE65000 also has an onboard programmable micro-controller for controlling the spectrometer and accessories. You have access to 10 user-programmable digital inputs/outputs and a pulse generator for triggering other devices. You can use the I/Os to flash a lamp, stop/start a process, and send a message/alarm during the spectrometer’s integration period. The spectrometer’s operating parameters can be controlled through software. In fact, wavelength calibration coefficients unique to each spectrometer are programmed into a memory chip right on the spectrometer.
The QE65000 interfaces to a computer via USB 2.0 or RS-232 serial port. Data unique to each spectrometer are programmed into a memory chip on the QE65000; our SpectraSuite Spectroscopy Operating Software reads these values for easy setup and hot swapping among computers, whether they run on Linux, Mac or Windows operating systems.
At Ocean Optics, we understand that your work is unique and that you want a spectrometer to fit your specific needs. With the QE65000 Spectrometer, you can configure the bench for your application. Choose QE65000 Optical Bench Options such as entrance aperture size, detector accessories, filters a grating and more. Our application scientists will help you determine which configuration will best work for you.
We have a number of variable bandpass order-sorting filter options available to vary the starting wavelength at 50 nm intervals to fit your particular application. These OFLVs are for use with the HC-1 grating only and consist of the following:
| OFLV Filter | Wavelength Range |
| OFLV-QE | 200-950 nm |
| OFLV-QE-250 | 250-1000 nm |
| OFLV-QE-300 | 300-1050 nm |
| OFLV-QE-350 | 350-1100 nm |
| OFLV-QE-400 | 400-1150 nm |
Our QE65000 Manual provides you with instructions for setting up, calibrating and performing experiments with your QE65000 spectrometer.
| Item | Description | Price |
| QE65000 | QE65000 User-configured Spectrometer. User to specify Optical Bench Options | $11549 |
| SpectraSuite | SpectraSuite Spectroscopy Operating Software | $199 |
|
Physical |
|
| Dimensions: | 182 mm x 110 mm x 47 mm |
| Weight: | 1.18 kg (without power supply) |
| Detector | |
|
Detector: |
Hamamatsu S7031-1006 |
| Detector range: | 200-1100 nm |
|
Pixels: |
1024 x 58 (1044 x 64 total pixels) |
|
Pixel size: |
24.576 μm2 |
|
Pixel well depth: |
1000 Ke- |
| Sensitivity: | ~0.065 counts / e- |
| Quantum efficiency: | 90% peak; 65% at 250 nm |
| Optical Bench | |
| Design: | f/4, Symmetrical crossed Czerny-Turner |
| Focal length: | 101.6 mm input and output |
| Entrance aperture: | 5, 10, 25, 50, 100 or 200 µm wide slits or fiber (no slit) |
| Grating options: | 14 different grating options, UV through Shortwave NIR |
| HC-1 grating option: | provides 200-950 nm range |
| Detector collection lens option: | None |
| OFLV filter options: | OFLV-QE (200-950 nm); OFLV-QE-250 (250-1000 nm); OFLV-QE-300 (300-1050 nm); OFLV-QE-350 (350-1100 nm); OFLV-QE-400 (400-1150 nm) |
| Other bench filter options: | Longpass OF-1 filters |
| Collimating and focusing mirrors: | Standard only |
| UV enhanced window: | No |
| Fiber optic connector: | SMA 905 to 0.22 numerical aperture single-strand optical fiber |
| Spectroscopic | |
| Wavelength range: | Grating dependent |
| Optical resolution: | ~0.14-7.7 nm FWHM |
| Signal-to-noise ratio: | 1000:1 (at full signal) |
| A/D resolution: | 16 bit |
| Dark noise: | 3 RMS counts |
| Dynamic range: | 7.5 x 109 (system), 25000:1 for a single acquisition |
| Integration time: | 8 ms to 15 minutes |
| Stray light: | <0.08% at 600 nm; 0.4% at 435 nm |
| Corrected linearity: | >99.8% |
| Electronics | |
| Power consumption: | 500 mA @ 5 VDC (no TE cooling); 3.5 A @ 5 VDC (with TE cooling) |
| Data transfer speed: | Full scans to memory every 7 ms with USB 2.0 port, 18 ms with USB1.1 port, 300 ms with serial port |
| Inputs/Outputs: | 10 onboard digital user-programmable GPIOs (general purpose inputs/outputs) |
| Analog channels: | No |
| Auto nulling: | Yes |
| Breakout box compatibility: | Yes, HR4-BREAKOUT |
| Trigger modes: | 4 modes |
| Strobe functions: | No |
| Gated delay feature: | Yes |
| Connector: | 30-pin connector |
| Power-up time: | <5 seconds |
|
Dark current: |
4000 e-/pixel/sec @ 25 ºC; 200 e-/pixel/sec @ 0 ºC |
| Computer | |
| Operating systems: | Windows 98/Me/2000/XP, Mac OS X and Linux with USB port; Any 32-bit Windows OS with serial port |
| Computer interfaces: | USB 2.0 @ 480 Mbps; RS-232 (2-wire) @ 115.2 K baud |
| Peripheral interfaces: | SPI (3-wire); I2C inter-integrated circuit |
| Temperature and Thermoelectric (TE) Cooling | |
| Temperature limits: | 0 ºC to 50.0 ºC; no condensation |
| Set point: | Software controlled; lowest set point is 40 ºC below ambient |
| Stability | +/-0.1 ºC of set temperature in <2 minutes |
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The QE65000
Scientific-grade Spectrometer is a novel combination
of detector, optical bench and electronics technologies that
provides users with a remarkably sensitive system for demanding
low-light level applications such as fluorescence, DNA sequencing,
astronomy and Raman spectroscopy. The QE65000 can achieve up to
90% quantum efficiency with high signal-to-noise and rapid signal
processing speed. The QE65000 Scientific-grade
Spectrometer is available for $11549.