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Home > Applications > Life Sciences > Protein & Nucleic Acid Analysis

Protein & Nucleic Acid Analysis

DNA Analysis

Ocean Optics offers spectrometers and accessories supporting a variety of sensing techniques for protein and nucleic acid analysis, including UV-Vis and NIR spectroscopy, Raman analysis and fluorescence. Our modular approach allows users to select from detector, optical bench and accessory options to configure systems for a wide range of applications.

Systems can be configured for a range of OEM and laboratory measurement needs, with applications such as DNA, RNA and protein determination among the options. The flexibility of our spectrometers and accessories allows users to switch easily among techniques such as absorbance and fluorescence.


Featured:

STS-UV Microspectrometer

STS-UV Microspectrometer

Compact yet powerful spectrometer is ideal for UV-Vis absorbance analysis of biological molecules.

Example Setup: Fluorescence of Biological Samples

Overview

Fluorescence measurements require a sensitive detector and an effective filter for discriminating between powerful excitation source wavelengths and weak emissions from the sample. For analysis of biological samples such as proteins, the amount of fluorescence can often give the researcher insight into the protein’s conformational states or activity under different biological conditions including changes in temperature, pH and ion concentration.

Fluorescence System Setup

We offer several spectrometers useful for fluorescence, but recommend the high-sensitivity, preconfigured QE Pro-FL for most fluorescence applications. The QE Pro-FL is set to ~350-1100 nm and is configured with a 200 µm slit and variable longpass filter.

In this setup, we use an LLS series UV LED for excitation. Nearly 20 different UV wavelength options are available, starting at 240 nm. The PX-2 pulsed xenon source is also useful for excitation.

Sampling Considerations

For solutions sampling, we use a CUV-ALL-UV 4-way cuvette holder, with the fibers coupled at 90 degrees to each other; 74-MSP mirrored screw plus to improve sensitivity; and two QP600-2-SR solarization-resistant optical fibers. Quartz cuvettes (1 cm pathlength) are used for the sample. Temperature-controlled cuvette holders are also available.

For most fluorescence experiments, you’ll need a filter to discriminate between excitation source wavelengths and spectral emissions from the sample. Our Linear Variable Filters are excellent for shaping the excitation energy from broadband sources. With a narrow-band excitation source such as an LED, a filter may not be necessary.

OceanView software completes the system.

Fluorescence System Components

The setup described here is just one of several options available for fluorescence measurement of solutions. For fluorescence of solids, one would likely select a broadband excitation source and a reflection/backscattering probe in place of the LED-cuvette holder setup described here.

Here are the items in the example setup:

QE Pro-FL High sensitivity, back thinned CCD spectrometer is preconfigured for fluorescence with 200 µm slit and order sorting filter
LLS-xxx LED for excitation is available in multiple UV wavelength options
CUV-ALL-UV 4-way cuvette holder for 1 cm pathlength cuvettes
74-MSP Mirror screw plugs for redirecting energy in the cuvette holder
QP600-2-SR 600 µm optical fiber, solarization resistant, 2 m length
CVFL-Q-10 Quartz cuvette, 1 cm pathlength, for fluorescence
OceanView Spectroscopy software

Also Notable

Ocean Optics provides the modular optical sensing solutions and applications know-how to help you solve your measurement problems with a great degree of flexibility. For example, when analyzing proteins, users can take advantage of our QE series spectrometer’s replaceable slit design to perform both fluorescence and absorbance measurements. Similarly, our 4-way cuvette holder can be positioned for both 90 degree (fluorescence) and straight-through (absorbance) measurements. This modular approach is not typically available in traditional lab instrumentation.

Fluorescence of Biological Samples

Absorbance characteristics of various proteins found in red blood cells

Absorbance characteristics of various proteins found in red blood cells.

Fluorescence spectra of Bovine Serum Albumin reveal changes in protein structure with exposure to a low-pH buffer.

Fluorescence spectra of Bovine Serum Albumin reveal changes in protein structure with exposure to a low-pH buffer.

Fluorescence measurements reveal lysozyme protein conformation changes.

Fluorescence measurements reveal lysozyme protein conformation changes.

Absorbance of NADH samples with quinine sulfate for comparison

Absorbance of NADH samples with quinine sulfate for comparison.

Protein & Nucleic Acid Analysis Protein & Nucleic Acid Analysis Protein & Nucleic Acid Analysis Protein & Nucleic Acid Analysis Protein & Nucleic Acid Analysis Protein & Nucleic Acid Analysis
OCEAN FX Spectrometers

OCEAN FX Spectrometers

Fast Acquisition Speed, Enhanced Communications
SERS Substrates

SERS Substrates

Surface Enhanced Raman Spectroscopy
Flame Spectrometer

Flame Spectrometer

High Thermal Stability, Interchangeable Slits
FLAME-BUNDLE-FL

FLAME-BUNDLE-FL

Application-Ready System for Fluorescence
FLAME-BUNDLE-ABS

FLAME-BUNDLE-ABS

Application-Ready System for Absorbance
Flame-NIR Spectrometer

Flame-NIR Spectrometer

Compact NIR Instrument
USB2000+ (Custom)

USB2000+ (Custom)

Custom Configured Spectrometer for Setup Flexibility
QE Pro (Custom)

QE Pro (Custom)

High-sensitivity Spectrometer for Low Light Level Applications
Maya2000 Pro (Custom)

Maya2000 Pro (Custom)

High Sensitivity Spectrometer
STS-UV

STS-UV

UV Spectral Analysis in a Tiny Footprint
USB4000 (Custom)

USB4000 (Custom)

Custom Configured for Maximum Flexibility
QE Pro-Raman

QE Pro-Raman

High-sensitivity Spectrometer for Raman
HR4000 (Custom)

HR4000 (Custom)

High Resolution Spectrometer for Maximum Flexibility
Ventana-785-Raman

Ventana-785-Raman

High-sensitivity Spectrometer for Raman Measurements
Maya2000 Pro-NIR

Maya2000 Pro-NIR

High-sensitivity Spectrometer for Raman and NIR Applications