Fluorescence Measurement With Spectrometers
Fluorescence is a high-sensitivity technique in which molecules typically absorb a lower wavelength of incident light from one direction and emit a higher wavelength of light in all directions. With a known incident light wavelength, samples can be identified by their fluorescent emission spectra.
Use Ocean Optics Spectrometers for Fluorescence Applications
Advantages
- High sensitivity – detection to single-molecule concentration levels
- Non-destructive – critical with small volume samples
- Quantitative results – ideal for reaction and process monitoring
Applications
- Analysis of rare earth salts
- Authentication of currency and documents, foods and fuels, and gemstones and luxury goods
- Clinical diagnostics for cancer detection, tissue health and vision screening
Fluorescence Measurement Setup
Here’s an example fluorescence system that uses a cuvette holder for sampling. To complete the system, you’ll need the spectrometer, an excitation source, optical fibers, and filters to block excitation energy. Multiple configurations are possible.
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