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Fluorescence Measurement
Fluorescence is the absorption and emission of light of two different frequencies. Typically, a lower wavelength of incident light is absorbed from one direction, and a higher wavelength of light is emitted in all directions.
Fluorescent molecules absorb light at a certain wavelength and emit at another. With a known incident light wavelength, samples can be identified by their fluorescent emission spectra. Because fluorescence occurs on a molecular scale, it is the only spectroscopic technique capable of identifying single molecules.
Advantages of Fluorescence
- High sensitivity - Very low sample concentration levels can be detected
- Quantitative results - In most cases, fluorescence signal is proportional to concentration and responds to concentration changes within picoseconds
- Non-destructive measurements - Unlike some techniques, fluorescence is non-destructive to both liquid and solid samples
Case Study & App Notes
Discover the remarkable sensitivity of Ocean ST in measuring the fluorescence of Europium chloride and Terbium chloride, rare earth salts used in products including EV batteries.
Related Products
With excellent response in the UV, the SR6 is a versatile spectrometer for measurement of biological macromolecules such as proteins and DNA. SR6 spectrometers offer high sensitivity and excellent SNR.
The QE Pro is a high sensitivity spectrometer for low light level applications including fluorescence, DNA sequencing and Raman analysis. Thermoelectric cooling provides thermal stability, and spectral buffering ensures data integrity at high collection rates.
